Project structure
Workgroups in the project
Genomics (Kmoch; 1.LF UK)
- Drives technological innovation in cost-effective, high-performance genome, transcriptome, and epigenome analysis.
- The National Center for Medical Genomics provides modern sequencing platforms (e.g., NovaSeqX, Oxford Nanopore, PacBio, AVITI).
- NCMG offers bioinformatics and statistical analysis to generate and interpret genetic data across individuals, families, and populations.
- Unlocks new insights into the molecular foundations of human health and disease.
Transcriptomics (Kubista; IBT)
- Develops methodologies for single-cell and spatial multi-omics analysis.
- Establishes robust, cost-effective, high-throughput workflows for comprehensive molecular multimarker profiling.
- Integrates digital sequencing to detect diagnostically relevant and rare gene variants.
- Includes tissue-specific methylation profiling, single-cell gene expression, microRNA profiling, and spatial transcriptomics.
- Enables precise biomarker discovery, disease mapping, and identification of novel therapeutic targets
Metabolomics (Kuchař; 1.LF UK)
- Develops and operates targeted and untargeted metabolomic and lipidomic methodologies.
- Analyzes small molecules in biological samples using advanced mass spectrometry and chromatography.
- Provides insights into chemical identity of metabolites, metabolic pathways, and their roles in health and disease.
- Compares molecular profiles between healthy and diseased individuals.
- Enables identification of biomarkers for diagnosis, disease monitoring, and evaluation of treatment effectiveness.
Multiomics BIOCEV
- Establishes a functionally complementary multi-omics technology platform.
- Enables academic and industry partners to enhance research quality on genetic and biological correlates of rare and common diseases.
- Supports targeted research and development of novel methodological, diagnostic, and therapeutic approaches.
- Ensures the transmission of research findings into clinical and laboratory practice.
Bioinformatics (Stránecký; 1.LF UK)
- Develops and applies innovative bioinformatics approaches for efficient genomic data processing and advanced statistical analyses.
- Designs automated and reproducible workflows for data analysis and interpretation from cutting-edge genomic technologies.
- Establishes and develops methods for processing MULTIOMICS data.
- Integrates genomic results with other omics layers for comprehensive analysis
Bioimaging (Pompach; AffiPro)
- Develops advanced methods to detect selected proteins and metabolites in tissue samples.
- Optimizes acquisition and analysis of microscopic data.
- Uses state-of-the-art imaging platforms to study protein localization, concentration, composition, and pathological forms.
- Identifies and analyzes protein biomarkers.
- Investigates cellular proteostasis and maps pathologically altered proteins and protein complexes
Proteomics (Novák; IMIC)
- Develops and operates targeted and untargeted proteomics methodologies.
- Validates detection and quantification of proteins as potential biomarkers.
- Designs final solutions for clinical laboratories and promotes new diagnostic workflows.
- Transmission of validated workflows into diagnostic kits for routine clinical use.
Providing expertise, people, and facilities
Participating institutions
Our team
People behind Multiomics
Prof. Ing. Stanislav Kmoch, CSc.
Institute for Inherited Metabolic Disorders, First Faculty of Medicine, Charles University in Prague
Prof. Kmoch is a biomedical scientist specializing in rare genetic diseases, directing national genomic infrastructures, and connecting research with biotechnology Innovation. He has developed and applied a broad spectrum of biochemical, genetic, molecular biology, genomic, next-generation sequencing, and bioinformatics methods, successfully identifying causal genes and characterizing the molecular and structural basis of over a dozen genetic disorders. His research has contributed to the development of novel diagnostic tools and therapeutic approaches..
More information can be found here.
prof. Dr. Mikael Kubista, Ph.D.
Laboratory of Gene Expression, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic
Prof. Kubista is a world-leading molecular diagnostics expert, an innovator of qPCR technologies, a founder of multiple innovative biotech companies that advance genomic diagnostics, molecular analysis, and ultrasensitive sequencing to support the development of advanced therapies through high-quality molecular analysis services.
More information can be found here.
prof. MUDr. Pavel Martásek, DrSc.
Scientific Director and Chairman of the BIOCEV Council
Prof. Martásek is a distinguished physician, scientist, and educator at the First Faculty of Medicine, Charles University, and Scientific Director of BIOCEV. Prof. Martásek has significantly contributed to the diagnosis and treatment of inherited metabolic disorders, nitrosative stress-related diseases and porphyria. He serves as an expert advisor for CUIP, Charles University’s technology transfer company, and holds multiple international patents in biomedicine and biotechnology.
More information can be found here.
RNDr. Petr Novák, Ph.D.
Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic.
RNDr. Novák is a proteomics and structural biology expert, a pioneer and innovator of protein mass spectrometry in the Czech Republic, directing research and infrastructure at the Czech Academy of Sciences and advancing protein analysis from clinical samples.
More information can be found here.
RNDr. Petr Pompach, Ph.D.
Institute of Biotechnology, The Czech Academy of Sciences, Vestec, Czech Republic
RNDr. Petr Pompach, Ph.D., is an expert in protein chemistry and mass spectrometry whose work advances molecular-level understanding of biological systems and clinical diagnostics. He heads the Mass Spectrometry Service at the Center of Molecular Structure and bridges academic research with industry, holding multiple publications and patents with significant impact on biotechnology.
Ing. Michal Pohludka, Ph.D., MBA, LL.M.
Genespector, Prague, Czech Republic
Ing. Pohludka is a Czech manager, investor, scientist, and entrepreneur with extensive leadership experience at Bio-Rad Laboratories. He co-founded the Charles University spin-off GeneSpector, which developed and commercialized a COVID-19 diagnostic kit and became the fastest-growing university spin-off in Czech history. He also founded Macromo, focusing on prevention of chronic diseases through genetic and lifestyle testing, and invests primarily in biomedical startups. He has served as Vice-Chairman of the Board of Charles University Innovations Prague, supporting technology transfer and commercialization across Czech universities.
More information can be found here.